MATERIALS1. Safety Needles, 22g or less
2. Butterfly needles. 21g or less
3. Syringes
4. Vacutainer tube holder
5. Transfer Device 6. Blood Collection Tubes.
The vacuum tubes are designed to draw a predetermined volume of blood.Tubes with different additives are used for collecting blood specimens for specific types of tests.The color of cap is used to identify these additives.7. Tourniquets. Single use, disposable, latex-free tourniquets
8. Antiseptic. Individually packaged 70% isopropyl alcohol wipes.
9. 2×2 Gauze
10. Sharps Disposal Container. An OSHA acceptable, puncture proof containermarked “Biohazardous”.
11. Bandages or tape
SAFETY1. Observe universal (standard) safety precautions.
2. Observe all applicable isolation procedures.
3. PPE’s will be worn at all times.
4. Wash hands in warm, running water with a appropriate hand washing product,
5. If hands are not visibly contaminated a commercial foaming hand wash product may be used before and after each patient collection.
6. Gloves are to be worn during all phlebotomies, and changed between patient collections.
7. Palpation of phlebotomy site may be performed without gloves providing the skin is not broken.
8. A lab coat or gown must be worn during blood collection procedures.
9. Needles and hubs are single use and are disposed of in an appropriate ‘sharps’ container as one unit.
10. Needles are never recapped, removed, broken, or bent after phlebotomy procedure.
11. Gloves are to be discarded in the appropriate container immediately after the phlebotomy procedure.
12. All other items used for the procedure must be disposed of according to proper biohazardous waste disposal policy.
13. Contaminated surfaces must be cleaned with freshly prepared 10% bleach solution. All surfaces are cleaned daily with bleach.
14. In the case of an accidental needlestick, immediately wash the area with an antibacterial soap, express blood from the wound, and contact your supervisor.
PROCEDURE
1. Identify the patient, two forms of active identification are required.
Ask the patient to state their name and date of birth.This information must match the requisition.2. Reassure the patient that the minimum amount of blood required for testing will be drawn.
3. Verify that any diet or time restrictions have been met.
4. Order of Draw
The following order of draw is the approved order as established by CLSI.This order of draw should be followed whenever multiple tubes are drawn during a single venipuncture.This is to prevent cross contamination by the tube additives that could lead to erroneous results.1. Blood Culture
2. Light Blue Top (plasma): 3.2% sodium citrate. These tubes are used for coagulation tests and need to be completely filled to ensure the proper ratio of blood to anticoagulant.
3. Red Top (serum): Plain and gel. Used for chemistry and reference tests.
4. Green Top (plasma): With and without gel, contains lithium heparin. These tubes are used primarily for chemistry tests.
5. Lavender or Pink Top (plasma): Contains EDTA. Used primarily for hematology and blood bank testing.
6. Gray Top (plasma): Contains sodium fluoride/potassium oxalate. Used by chemistry for glucose testing.
7. Yellow Top (plasma and cells): Contains ACD solution A or B. Used for Genetics testing. all products